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RF levels in the overexpression HEK293T cell lines. ( A ). Quantification of RF mRNA expression in the overexpressing cell lines and control cells (Ctl) by qRT-PCR. Because of their very similar sequences (93,3% identity), IFITM2 and IFITM3 transcript levels were measured with the same primer pair. The results correspond to the means and standard deviations of two RNA extractions, each of them measured in duplicates. ( B ). Expression of IFITM proteins in the overexpressing cell lines detected by western blot. <t>IFITM1</t> (Top) and IFITM2/3 (Bottom) were tested independently in all 6 cell lines and were detected only in the corresponding overexpressing cell lines (apparent MW for both 17 kDa, top panels). Total protein content in each well was checked by looking at actin content (MW 46 kDa, lower panels).
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https://www.bioz.com/result/ifitm1 antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
ifitm1 antibody - by Bioz Stars, 2026-03
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RF levels in the overexpression HEK293T cell lines. ( A ). Quantification of RF mRNA expression in the overexpressing cell lines and control cells (Ctl) by qRT-PCR. Because of their very similar sequences (93,3% identity), IFITM2 and IFITM3 transcript levels were measured with the same primer pair. The results correspond to the means and standard deviations of two RNA extractions, each of them measured in duplicates. ( B ). Expression of IFITM proteins in the overexpressing cell lines detected by western blot. <t>IFITM1</t> (Top) and IFITM2/3 (Bottom) were tested independently in all 6 cell lines and were detected only in the corresponding overexpressing cell lines (apparent MW for both 17 kDa, top panels). Total protein content in each well was checked by looking at actin content (MW 46 kDa, lower panels).
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Average 94 stars, based on 1 article reviews
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RF levels in the overexpression HEK293T cell lines. ( A ). Quantification of RF mRNA expression in the overexpressing cell lines and control cells (Ctl) by qRT-PCR. Because of their very similar sequences (93,3% identity), IFITM2 and IFITM3 transcript levels were measured with the same primer pair. The results correspond to the means and standard deviations of two RNA extractions, each of them measured in duplicates. ( B ). Expression of IFITM proteins in the overexpressing cell lines detected by western blot. IFITM1 (Top) and IFITM2/3 (Bottom) were tested independently in all 6 cell lines and were detected only in the corresponding overexpressing cell lines (apparent MW for both 17 kDa, top panels). Total protein content in each well was checked by looking at actin content (MW 46 kDa, lower panels).

Journal: Scientific Reports

Article Title: Combined effects of restriction factors and transduction adjuvants on lentiviral vector gene transfer efficacy

doi: 10.1038/s41598-025-22470-9

Figure Lengend Snippet: RF levels in the overexpression HEK293T cell lines. ( A ). Quantification of RF mRNA expression in the overexpressing cell lines and control cells (Ctl) by qRT-PCR. Because of their very similar sequences (93,3% identity), IFITM2 and IFITM3 transcript levels were measured with the same primer pair. The results correspond to the means and standard deviations of two RNA extractions, each of them measured in duplicates. ( B ). Expression of IFITM proteins in the overexpressing cell lines detected by western blot. IFITM1 (Top) and IFITM2/3 (Bottom) were tested independently in all 6 cell lines and were detected only in the corresponding overexpressing cell lines (apparent MW for both 17 kDa, top panels). Total protein content in each well was checked by looking at actin content (MW 46 kDa, lower panels).

Article Snippet: Membranes were blotted with anti IFITM1 antibody (Proteintech 60,074–1-Ig, 1/5000) or anti IFITM2/3 antibody (Proteintech 66,081–1-Ig, 1/5000).

Techniques: Over Expression, Expressing, Control, Quantitative RT-PCR, Western Blot